Helicase Enzyme: DNA Unwinding, Replication Fork and Genome Stability

UPSC Relevance: Biotechnology | Genetics

Why in News?

A 2026 study examined molecular controls that prevent premature activation of helicase during DNA replication.

What is Helicase?

An enzyme and molecular motor that unwinds double-stranded nucleic acids.

During DNA Replication

Helicase:

  1. Binds DNA.

  2. Uses energy generated from ATP hydrolysis.

  3. Breaks hydrogen bonds between complementary bases.

  4. Separates DNA strands.

  5. Creates the replication fork.

Important Concept

Helicase works alongside:

  • DNA polymerase.

  • Topoisomerase.

  • Primase.

  • Single-strand binding proteins.

Prelims Pointer: Helicase unwinds DNA; DNA polymerase synthesizes DNA.